top of page
Antibody System Recombinant Human PDGFA Protein, N-His

Antibody System Recombinant Human PDGFA Protein, N-His

Product Overview
Advance your cell biology and tissue regeneration research with the Antibody System Recombinant Human PDGFA Protein (N-His). Platelet-Derived Growth Factor Subunit A (PDGFA) is a classic mitogenic factor that plays a crucial role in embryonic development, cell proliferation, migration, and angiogenesis. It acts as a potent chemoattractant and mitogen for mesenchymal cells, including fibroblasts and smooth muscle cells.
Engineered with an N-terminal His-tag and produced via an efficient E. coli expression system, this recombinant protein provides a consistent, high-yield solution for evaluating mesenchymal signaling networks, wound healing models, and fibroproliferative mechanisms.
 
DATASHEET

  • PRODUCT INFO

    Key Features & Specifications

    • High-Yield Expression System: Produced in E. coli to ensure excellent batch-to-batch reproducibility and optimal yield consistency.

    • High Purity Profile: Verified at >90% purity using SDS-PAGE analysis, ensuring minimal background interference in sensitive assays.

    • Advanced Lyophilization Matrix: Formulated in a protective solution of PBS (pH 7.4), 0.02% NLS, 1mM EDTA, 4% Trehalose, and 1% Mannitol to secure long-term structural stability.

    • Multi-Assay Compatibility: Validated for use in ELISA, Western Blotting (WB), SDS-PAGE, and immunogen preparation. Bioactivity testing is actively in progress.

    Technical Specifications

    Feature Details
    Catalog No. YHC06301
    Species Homo sapiens (Human)
    Expression System E. coli
    Accession Number P04085
    Sequence Range Ser87–Thr211
    Predicted Mol. Weight 16.62 kDa
    Form Lyophilized powder
    Endotoxin Level Available upon request (Contact Lab)

    Alternative Names: PDGFA, PDGF-1, PDGF1, PDGF subunit A, Platelet-derived growth factor alpha polypeptide, Platelet-derived growth factor subunit A, Platelet-derived growth factor A chain.

    Biological Context & Research Applications

    PDGFA functions as either a disulfide-linked homodimer (PDGF-AA) or a heterodimer with PDGFB (PDGF-AB), signaling through the PDGF receptor tyrosine kinases. It is highly relevant for investigating:

    • The molecular mechanisms behind cell proliferation, migration, and survival in mesenchymal lineages.

    • Pathways regulating wound healing, tissue remodeling, and fibrotic disease progression.

    • The design, screening, and validation of targeted therapeutics and inhibitors in fibroproliferative disorders and oncology.

    Handling, Reconstitution & Storage

    • Shipping: Shipped under strictly controlled conditions on blue ice or dry ice to ensure maximum stability upon delivery.

    • Reconstitution: Reconstitute using sterile water to create a stable primary stock solution. A product-specific technical datasheet will accompany the shipment for exact configuration details.

    • Storage Framework: * Short-Term: Store at 2°C to 8°C for regular, frequent application.

      • Long-Term: Store at -20°C to -80°C for up to 12 months from the date of receipt.

      • Note: Use a manual defrost freezer and avoid repeated freeze-thaw cycles to maximize product performance.

Heading 6

Related Products

bottom of page