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Antibody System Recombinant Mouse IL25 Protein, N-His

Antibody System Recombinant Mouse IL25 Protein, N-His

Product Overview
Accelerate your mucosal immunity and Type 2 inflammation research with the Antibody System Recombinant Mouse IL25 Protein (N-His). Interleukin-25 (IL-25)—also known as Interleukin-17E (IL-17E)—is a distinct member of the IL-17 cytokine family. Secreted primarily by specialized epithelial cells, such as tuft cells, IL-25 functions as a powerful upstream initiator of the Th2 immune response, driving eosinophil infiltration, airway hyperreactivity, and protective immunity against helminth infections.
Expressed in mammalian cells to ensure authentic post-translational modifications and correct structural folding, this recombinant protein features an N-terminal His-tag for reliable purification, detection, and tracking throughout your in vitro and in vivo murine experimental models.
 
 
DATASHEET

  • PRODUCT INFO

    Key Features & Specifications

    • Mammalian Expression System: Produced in mammalian cells to guarantee native-like conformational architecture, proper disulfide bonding, and premium biological relevance.

    • Premium Purity Standards: Verified at >90% purity via SDS-PAGE evaluation, minimizing background interference and ensuring highly reproducible results.

    • Optimized Lyophilization Formulation: Meticulously stabilized in a solution of PBS (pH 7.4) supplemented with 5% Trehalose and 5% Mannitol to secure optimal cake formation and structural integrity.

    • Versatile Assay Integration: Fully compatible with ELISA, Western Blotting (WB), SDS-PAGE, and immunogen preparation. Bioactivity testing is actively in progress.

    Technical Specifications

    Feature Details
    Catalog No. EMJ55501
    Species Mus musculus (Mouse)
    Expression System Mammalian Cells
    Accession Number Q8VHH8
    Sequence Range Val17–Ala169
    Predicted Mol. Weight 20 kDa
    Form Lyophilized powder
    Endotoxin Level Available upon request (Contact Lab)

    Alternative Names: IL-25, IL25, Il25, Interleukin-25, Interleukin-17E, IL-17E, Il17e.

    Biological Context & Research Applications

    IL-25 signals via a heterodimeric receptor complex composed of IL-17RA and IL-17RB, inducing downstream activation of the NF-$\kappa$B and MAPK pathways. It acts as a primary coordinator at mucosal barriers, making this recombinant mouse protein an indispensable tool for investigating:

    • The polarization and activation of Type 2 innate lymphoid cells (ILC2s), Th2 cells, and mast cells.

    • The molecular pathogenesis of allergic asthma, atopic dermatitis, and chronic inflammatory bowel diseases.

    • Host-pathogen defense mechanisms, specifically mucosal immune responses during parasitic helminth infections.

    Handling, Reconstitution & Storage

    • Shipping: Distributed under temperature-controlled conditions on blue ice or dry ice to safeguard protein viability upon arrival.

    • Reconstitution: Reconstitute using sterile water to establish a stable, concentrated stock solution.

    • Storage Framework:

      • Short-Term: Store at 2°C to 8°C for routine, frequent laboratory use.

      • Long-Term: Store at -20°C to -80°C for up to 12 months from the date of receipt.

      • Note: Utilize a manual defrost freezer and avoid repeated freeze-thaw cycles to prevent loss of protein activity.

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