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Antibody System Recombinant Human CTGF/CCN2 Protein, Tag-Free

Antibody System Recombinant Human CTGF/CCN2 Protein, Tag-Free

Product Overview
Accelerate your extracellular matrix, tissue remodeling, and fibrosis research with the Antibody System Recombinant Human CTGF/CCN2 Protein (Tag-Free). Connective Tissue Growth Factor (CTGF)—officially designated as CCN family member 2 (CCN2)—is a highly conserved matricellular protein that acts as a central coordinator of cellular adhesion, migration, proliferation, and extracellular matrix (ECM) synthesis. It operates downstream of TGF-beta to heavily influence chondrogenesis, angiogenesis, and fibrotic cascades.
 
This recombinant protein is expressed in mammalian cells to ensure authentic conformational folding and native post-translational modifications. Formulated entirely tag-free, it features an unhindered amino acid sequence that prevents potential steric hindrance or non-specific interactions typically caused by artificial fusion tags, making it highly suitable for precise structural and functional assays.
 
 
DATASHEET

  • PRODUCT INFO

    Key Features & Specifications

    • Tag-Free Configuration: Purified in its native structural form without any fusion tags, guaranteeing completely unhindered receptor-ligand engagement.

    • Premium Purity & Quality: Verified at an exceptional >95% purity via SDS-PAGE evaluation, delivering excellent batch-to-batch consistency and minimizing experimental background.

    • Low Endotoxin Footprint: Stringently validated at <1 EU/µg using the LAL assay, ensuring minimal risk of endotoxin-induced cell toxicity or non-specific immune activation.

    • Mammalian Cell Expression: Produced in a mammalian expression system to secure authentic glycosylation, disulfide bonding patterns, and biological relevance.

    Technical Specifications

    Feature Details
    Catalog No. EHD82303
    Species Homo sapiens (Human)
    Expression System Mammalian Cells
    Accession Number Q5M8T4
    Sequence Range Gln27–Ala180
    Predicted Mol. Weight 16.3 kDa
    Form Lyophilized powder
    Storage Buffer Lyophilized from a 0.22 $\mu$m filtered solution in PBS (pH 7.4)

    Alternative Names: CTGF, CCN2, CCN family member 2, Connective tissue growth factor, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8.

    Biological Context & Research Applications

    CTGF interacts with a broad array of cell surface molecules, including integrins, heparan sulfate proteoglycans, and cytokine receptors, to regulate skeletal development and tissue repair. This tag-free human recombinant protein is ideal for investigating:

    • The molecular pathogenesis of fibroproliferative diseases, such as pulmonary, hepatic, and renal fibrosis.

    • Chondrocyte differentiation, osteogenesis, and cartilage repair mechanisms.

    • Tumor-stroma interactions, angiogenesis, and cell adhesion remodeling within tumor microenvironments.

    Handling, Reconstitution & Storage

    • Shipping: Shipped under temperature-controlled conditions on blue ice or dry ice to maximize protein integrity upon delivery.

    • Reconstitution: Reconstitute using sterile water to establish a stable primary stock solution.

    • Storage Framework:

      • Short-Term: Store at 2°C to 8°C for up to one week of routine, frequent laboratory use.

      • Long-Term: Store at -20°C to -80°C for up to 12 months from the date of receipt.

      • Note: Utilize a manual defrost freezer and avoid repeated freeze-thaw cycles to keep the protein at peak performance.

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