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Antibody System Recombinant Cat FGF7/KGF Protein, C-His

Antibody System Recombinant Cat FGF7/KGF Protein, C-His

Product Overview
Advance your feline epithelial biology, wound healing, and tissue remodeling research with the Antibody System Recombinant Cat FGF7/KGF Protein (C-His). Fibroblast Growth Factor 7 (FGF7)—also widely designated as Keratinocyte Growth Factor (KGF)—is a potent, paracrine-acting mitogen secreted predominantly by mesenchymal cells. It targets epithelial cells with high specificity, signaling through the FGFR2b receptor splice variant to direct critical cellular proliferation, migration, and differentiation programs.
Expressed in mammalian cells to ensure optimal conformational folding and native-like post-translational modifications, this recombinant protein features a C-terminal His-tag for streamlined tracking and purification, delivering superior reliability for feline-specific cell culture and functional models.
 
 
DATASHEET

  • PRODUCT INFO

    Key Features & Specifications

    • Mammalian Expression System: Produced in mammalian cells to guarantee native-like conformational architecture, glycosylation patterns, and maximum physiological relevance.

    • High Purity Profile: Verified at >90% purity using SDS-PAGE analysis, ensuring reproducible experimental results with minimal assay background interference.

    • Enhanced Lyophilization Matrix: Meticulously stabilized in a protective formulation of PBS (pH 7.4), 1mM EDTA, 4% Trehalose, and 1% Mannitol to secure long-term structural integrity.

    • Versatile Assay Compatibility: Fully optimized for integration into ELISA, Western Blotting (WB), SDS-PAGE, and immunogen preparation workflows. Bioactivity testing is currently underway.

    Technical Specifications

    Feature Details
    Catalog No. EFD47701
    Species Felis catus (Cat)
    Expression System Mammalian Cells
    Accession Number A0ABI7YB69
    Sequence Range Cys74–Thr236
    Predicted Mol. Weight 20.86 kDa
    Form Lyophilized powder
    Endotoxin Level Available upon request (Contact Lab)

    Alternative Names: FGF7, FGF-7, KGF, Keratinocyte growth factor, Fibroblast growth factor 7, Heparin-binding growth factor 7, HBGF-7.

    Biological Context & Research Applications

    FGF7 acts as a fundamental coordinator of epithelial barrier homeostasis. Because its activity is restricted strictly to epithelial cells, this recombinant cat protein serves as an essential tool for evaluating:

    • Feline corneal ulceration, skin injury models, and mucosal wound healing acceleration.

    • Protective mechanisms and inflammatory responses in feline lung, gastrointestinal, and renal epithelial tissues.

    • Comparative veterinary medicine investigating mesenchymal-epithelial interactions in chronic disease pathologies.

    Handling, Reconstitution & Storage

    • Shipping: Distributed under temperature-controlled conditions on blue ice or dry ice to safeguard protein viability upon arrival.

    • Reconstitution: Reconstitute using sterile water to prepare a stable primary stock solution.

    • Storage Framework:

      • Short-Term: Store at 2°C to 8°C for up to one week of routine, frequent laboratory use.

      • Long-Term: Store at -20°C for up to 12 months from the date of receipt.

      • Note: Use a manual defrost freezer and avoid repeated freeze-thaw cycles to protect the protein from activity loss.

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