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Antibody System Recombinant Cat HGF Protein, C-His

Antibody System Recombinant Cat HGF Protein, C-His

Product Overview

Accelerate your veterinary medicine, tissue regeneration, and oncology research with the Antibody System Recombinant Cat HGF Protein (C-His). Hepatocyte Growth Factor (HGF)—also known as Scatter Factor (SF)—is a pleiotropic cytokine that plays a foundational role in cell proliferation, migration, survival, and morphogenesis across various epithelial and endothelial lineages.

Expressed in mammalian cells to guarantee native-like post-translational modifications and accurate conformational folding, this high-molecular-weight recombinant protein features a C-terminal His-tag for reliable tracking, purification, and detection, making it an indispensable tool for robust feline functional assays.

 

 

DATASHEET

  • PRODUCT INFO

    Key Features & Specifications

    • Mammalian Expression System: Produced in mammalian cells to ensure authentic glycosylation, proteolytic processing, and optimal physiological relevance.

    • Premium Purity Standards: Verified at >90% purity via SDS-PAGE analysis, delivering reliable, reproducible assay outcomes with minimal background interference.

    • Advanced Lyophilization Formulation: Stabilized in a balanced mixture of PBS (pH 7.4), 1mM EDTA, 4% Trehalose, and 1% Mannitol to secure long-term structural integrity.

    • Multi-Assay Compatibility: Validated for use in ELISA, Western Blotting (WB), SDS-PAGE, and immunogen preparation. Bioactivity testing is actively in progress.

    Technical Specifications

    Feature Details
    Catalog No. EFD03701
    Species Felis catus (Cat)
    Expression System Mammalian Cells
    Accession Number Q9BH09
    Sequence Range Gln30–Ser728
    Predicted Mol. Weight 81.75 kDa
    Form Lyophilized powder
    Endotoxin Level Available upon request (Contact Lab)

    Alternative Names: HGF, SF, HPTA, Hepatocyte growth factor, Scatter factor, Hepatopoietin-A.

    Biological Context & Research Applications

    HGF exerts its biological behavior by binding to the c-Met receptor tyrosine kinase, initiating signaling cascades critical for cellular repair and oncology profiles. In feline research, this axis is heavily investigated for:

    • Feline chronic kidney disease (CKD) pathology and renal tissue regeneration.

    • Hepatic regeneration, wound healing acceleration, and tissue remodeling models.

    • Feline oncology pathways, specifically investigating c-Met dysregulation in carcinomas and sarcomas.

    Handling, Reconstitution & Storage

    • Shipping: Distributed under temperature-controlled conditions on blue ice or dry ice to safeguard protein viability upon arrival.

    • Reconstitution: Reconstitute using sterile water to create a stable primary stock solution.

    • Storage Framework: * Short-Term: Store at 2°C to 8°C for up to one week of routine, frequent laboratory use.

      • Long-Term: Store at -20°C for up to 12 months from the date of receipt.

      • Note: Use a manual defrost freezer and avoid repeated freeze-thaw cycles to prevent loss of protein activity.

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