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Antibody System Recombinant Human CCL18/PARC/MIP-4 Protein, N-His

Antibody System Recombinant Human CCL18/PARC/MIP-4 Protein, N-His

Product Overview
Optimize your immunology and oncology workflows with the Antibody System Recombinant Human CCL18/PARC/MIP-4 Protein (N-His). Chemokine (C-C motif) ligand 18 (CCL18)—also widely recognized as PARC or MIP-4—is a chemotactic factor predominantly expressed by macrophages, dendritic cells, and alternative (M2) activated macrophages. It plays a foundational role in leukocyte trafficking, tissue remodeling, and modulating the tumor microenvironment.
Engineered with an N-terminal His-tag and produced using a robust E. coli expression system, this recombinant protein provides a highly consistent, premium-grade solution for analyzing macrophage polarization, cellular chemotaxis, and fibrotic signaling pathways.
 
DATASHEET

  • PRODUCT INFO

    Key Features & Specifications

    • High-Yield Expression System: Expressed in E. coli to deliver exceptional batch-to-batch reproducibility and a highly concentrated yield.

    • Premium Purity Standards: Verified at >90% purity via SDS-PAGE evaluation, ensuring reliable assay outcomes with minimal background interference.

    • Advanced Lyophilization Formulation: Stabilized in a balanced mixture of PBS (pH 7.4), 0.02% NLS, 1mM EDTA, 4% Trehalose, and 1% Mannitol to secure structural stability.

    • Broad Application Compatibility: Tailored for use in ELISA, Western Blotting (WB), SDS-PAGE, and immunogen preparation. Bioactivity testing is currently in progress.

    Technical Specifications

    Feature Details
    Catalog No. YHF13501
    Species Homo sapiens (Human)
    Expression System E. coli
    Accession Number P55774
    Sequence Range Ala21–Ala89
    Predicted Mol. Weight 10.15 kDa
    Form Lyophilized powder
    Endotoxin Level Available upon request (Contact Lab)

    Alternative Names: CCL18, PARC, MIP-4, MIP4, AMAC1, AMAC-1, DC-CK1, DCCK1, Small-inducible cytokine A18, C-C motif chemokine 18, Pulmonary and activation-regulated chemokine, Alternative macrophage activation-associated CC chemokine 1, Macrophage inflammatory protein 4, Dendritic cell chemokine 1, SCYA18.

    Biological Context & Research Applications

    CCL18 is highly involved in managing adaptive immune responses and is primarily chemotactic for naive T cells, T regulatory cells, and B cells. Because of its intense upregulation by M2 macrophages, it serves as a central point of interest in investigating:

    • The molecular architecture driving tumor-associated macrophage (TAM) infiltration and tumor progression.

    • Fibrotic lung diseases, including idiopathic pulmonary fibrosis (IPF) and systemic sclerosis.

    • Chronic inflammatory diseases and alternative macrophage activation pathways.

    Handling, Reconstitution & Storage

    • Shipping: Safely distributed under temperature-controlled conditions on blue ice or dry ice to maximize protein integrity.

    • Reconstitution: Reconstitute with sterile water to establish a stable, concentrated stock solution.

    • Storage Protocols:

      • Short-Term: Store at 2°C to 8°C for immediate, frequent laboratory use.

      • Long-Term: Store at -20°C to -80°C for up to 12 months from the date of receipt.

      • Note: Utilize a manual defrost freezer and avoid repeated freeze-thaw cycles to keep the protein at peak performance.

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