Antibody System Research Grade Sotigalimab
Overview
This research-grade monoclonal antibody is engineered to selectively target and activate Human Cluster of Differentiation 40 (CD40), also recognized as Tumor Necrosis Factor Receptor Superfamily Member 5 (TNFRSF5). CD40 is a key co-stimulatory receptor expressed prominently on antigen-presenting cells (APCs), including dendritic cells, B lymphocytes, and macrophages. Binding of an agonist antibody to CD40 mimics the structural interactions of the native CD40 ligand (CD40L), triggering robust antigen presentation pathways, upregulating secondary co-stimulatory molecules, and driving the secretion of pro-inflammatory cytokines. This coordinated cellular cascade effectively bridges the innate and adaptive immune axes, inducing a multifaceted immune response that activates cytotoxic T-cell networks to overcome tumor-mediated immunosuppression. Developed as a high-affinity research-grade biosimilar based on clone APX005M, this humanized monoclonal antibody serves as a vital analytical instrument for tumor immunologists and pharmacologists analyzing myeloid cell activation mechanics, mapping checkpoint combination synergy, and evaluating targeted immunotherapies across diverse solid tumor models, including soft tissue sarcomas.
PRODUCT INFO
Key Features and Performance Metrics
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Refined Purity Profile: Exhibits an exceptional structural purity exceeding 95 percent, quantified and verified via Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
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Uniform Concentration Structure: Formulated at a precise stock concentration of 1 mg/ml to seamlessly accommodate routine assay optimization and titration setups.
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Humanized Monoclonal Precision: Engineered as an IgG1-Kappa assembly from an advanced mammalian cell expression platform, ensuring native-like post-translational modifications, uniform receptor cross-linking kinetics, and minimized anti-species background.
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Validated Upstream Recovery: Isolated and polished via specialized Protein A/G affinity chromatography directly from cell culture supernatants to minimize processing contaminants.
Technical Specifications
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Catalog No.: DHD68911
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Targeted Biomarker: CD40 (Tumor Necrosis Factor Receptor Superfamily Member 5 / TNFRSF5)
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Host Species: Humanized
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Clonality: Monoclonal
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Clone ID: Sotigalimab (APX005M)
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Isotype: IgG1-Kappa
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Species Reactivity: Human
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Accession Number: P25942
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Endotoxin Level: Batch-specific; please contact technical services for current certificate of analysis metrics.
Alternative Names: APX005M, APX-005, APX-005-M, APX-005M, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CAS: 2305607-45-6.
Applications and Workflow Summary
This humanized monoclonal antibody is highly optimized for performance as a research-grade biosimilar in downstream preclinical models. Investigators should observe the following guidelines:
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Dilution and Layout: Optimal operational concentrations should be determined empirically by the investigator based on specific assay layouts (e.g., cell-based APC maturation assays, T-cell activation screens, binding ELISA, or surface plasmon resonance configurations).
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Analysis Requirements: To avoid physical shear stress or structural degradation, always handle stock aliquots with care and centrifuge the vial briefly before opening to collect the liquid contents.
Handling, Stability and Storage
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Liquid Formulation: Supplied in a highly stable liquid format dissolved in 0.01M Phosphate Buffered Saline (PBS), pH 7.4.
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Storage Guidance:
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Short-Term Storage: Maintain at 4°C for standard short-term use (1 to 2 weeks).
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Long-Term Storage: For intermediate storage up to 12 months, keep at -20°C. For extended long-term preservation, store at -80°C.
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Operational Shelf Life: To prevent structural fragmentation and preserve structural binding capability, use a manual defrost freezer and strictly avoid repeated freeze-thaw cycles.
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