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Antibody System Research Grade Anti-Human FGF19 (1A6)

Antibody System Research Grade Anti-Human FGF19 (1A6)

Overview
This research-grade monoclonal antibody is engineered to selectively bind to Human Fibroblast Growth Factor 19 (FGF19). FGF19 is an endocrine gastrointestinal hormone belonging to the FGF15/19 subfamily, which mediates downstream physiological responses by binding to the FGF receptor 4 (FGFR4) in complex with the β-Klotho co-receptor. It acts as a primary homeostatic regulator of bile acid synthesis, gallbladder filling, glycogen storage, and metabolic rate within the enterohepatic axis. Beyond its standard physiological roles, aberrant activation of the FGF19/FGFR4 signaling axis has been implicated in driving hepatocyte proliferation, hepatocellular carcinoma (HCC), and metabolic disorders. Developed as a high-affinity research-grade biosimilar, this monoclonal antibody serves as a vital analytical instrument for clinical investigators, oncologists, and pharmacologists analyzing receptor-ligand neutralizing interfaces, mapping cell proliferation pathways, and verifying downstream metabolic cascade variations in preclinical drug validation pipelines.
 
DATASHEET

  • PRODUCT INFO

    Key Features and Performance Metrics

    • Refined Purity Profile: Exhibits an exceptional structural purity exceeding 95 percent, quantified and verified via Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).

    • Uniform Concentration Structure: Formulated at a precise stock concentration of 1 mg/ml to seamlessly accommodate routine assay optimization and titration setups.

    • Targeted Precision Layer: Expressed as a monoclonal IgG assembly from an advanced mammalian cell expression platform, ensuring native-like post-translational modifications and reproducible binding affinity.

    • Validated Upstream Recovery: Isolated and polished via specialized Protein A/G affinity chromatography directly from cell culture supernatants to minimize processing contaminants.

    Technical Specifications

    • Catalog No.: DHB73301

    • Targeted Biomarker: Fibroblast Growth Factor 19 (FGF19 / FGF-19)

    • Host Species: Human

    • Clonality: Monoclonal

    • Clone ID: U3-1784

    • Isotype: Human IgG

    • Species Reactivity: Human

    • Alternative Names: U3-1784

    • Accession Number: O95750

    • Endotoxin Level: Batch-specific; please contact technical services for current certificate of analysis metrics.

    Applications and Workflow Summary

    This human monoclonal antibody is highly optimized for performance as a research-grade biosimilar in downstream preclinical models. Investigators should observe the following guidelines:

    • Dilution and Layout: Optimal operational concentrations should be determined empirically by the investigator based on specific assay layouts (e.g., cell-based neutralizing assays, binding ELISA, surface plasmon resonance configurations).

    • Analysis Requirements: To avoid physical shear stress or structural degradation, always handle stock aliquots with care and centrifuge the vial briefly before opening to collect the liquid contents.

    Handling, Stability and Storage

    • Liquid Formulation: Supplied in a highly stable liquid format dissolved in 0.01M Phosphate Buffered Saline (PBS), pH 7.4.

    • Storage Guidance:

      • Short-Term Storage: Maintain at 4°C for standard short-term use (1 to 2 weeks).

      • Long-Term Storage: For intermediate storage up to 12 months, keep at -20°C. For extended long-term preservation, store at -80°C.

    • Operational Shelf Life: To prevent structural fragmentation and preserve structural binding capability, use a manual defrost freezer and strictly avoid repeated freeze-thaw cycles.

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