top of page
Antibody System Anti-HAdV-5 L3 / CP-H / Protein II Antibody (9C12)

Antibody System Anti-HAdV-5 L3 / CP-H / Protein II Antibody (9C12)

Overview
This monoclonal antibody specifically targets the hexon protein, also recognized as Protein II, CP-H, or L3, which is the major structural capsid protein of Human Adenovirus C serotype 5 (HAdV-5). The hexon protein is the most abundant component of the non-enveloped adenoviral capsid, forming 240 trimeric facets that dictate the structural integrity and geometric stability of the virion. Beyond its structural role, hexon is a primary target for host immune neutralization and mediates critical interactions with host cell surface molecules, including coagulation factors like Factor X, which facilitate viral trafficking to the liver. Because HAdV-5 is widely engineered as a prominent vector for gene therapy, vaccine delivery, and oncolytic virology, tracking its structural assembly and vector distribution is paramount. Generated from mouse hybridoma clone 9C12, this purified IgG1 kappa monoclonal antibody delivers high-affinity, specific target recognition for Human Adenovirus 5, providing a rigorous and highly reproducible tool for evaluating viral replication, vector clearance, and quality-control manufacturing of adenoviral platforms.
 
DATASHEET

  • PRODUCT INFO

    Key Features and Specifications

    • Monoclonal Precision: Engineered from mouse clone 9C12 to deliver highly uniform binding kinetics, low background cross-reactivity, and absolute specificity for the adenoviral hexon structural subunit.

    • Vector and Vaccine Quality Tool: Serves as an excellent diagnostic reagent for monitoring vector distribution, quantifying viral titers, and verifying capsid integrity in recombinant HAdV-5 platforms.

    • High Purity and Yield: Verified at greater than 95% purity via SDS-PAGE evaluation, achieved through advanced Protein A/G affinity chromatography from cell culture supernatant.

    • Versatile Assay Performance: Validated across cornerstone laboratory methodologies, including Western Blotting (WB), Immunohistochemistry (IHC), and Enzyme-Linked Immunosorbent Assays (ELISA).

    • High-Stability Liquid Matrix: Supplied at a high concentration of 3.25 mg/ml in a ready-to-use 0.01M PBS (pH 7.4) matrix to minimize formulation interference in downstream screening assays.

    Technical Specifications

    • Catalog No.: RVV04401

    • Host Species: Mouse

    • Clone ID: 9C12

    • Clonality: Monoclonal

    • Isotype: IgG1, kappa

    • Target Protein: Hexon protein (Protein II / CP-H / L3)

    • Accession Number: P04133

    • Species Reactivity: Human adenovirus C serotype 5 (HAdV-5)

    • Concentration: 3.25 mg/ml

    • Purification: Protein A/G chromatography

    • Form: Liquid solution

    • Storage Buffer: 0.01M PBS, pH 7.4

    Alternative Names: Hexon protein, Protein II, CP-H, L3, Major capsid protein, Adenovirus 5 hexon.

    Applications and Recommended Dilutions

    This mouse monoclonal IgG1 antibody is highly suited for running quantitative, localized, and profile expression screens within adenoviral vector development and pathogenesis workflows. Investigators should utilize the following initial dilution guidelines:

    • Western Blotting (WB): 1:2000 to 1:8000 dilution for resolving target hexon expression bands or tracking structural capsid assembly processing under denaturing or non-denaturing conditions.

    • Enzyme-Linked Immunosorbent Assay (ELISA): 1:4000 to 1:8000 dilution for target capture validation, solid-phase sandwich screening assemblies, or viral particle titration panels.

    • Immunohistochemistry (IHC): 1:50 to 1:100 dilution for high-resolution spatial mapping of viral protein localization and tissue distribution within infected or transduced cell layers and tissue sections.

    • Note: Optimal working dilutions must be determined empirically by the end-user according to specific sample viral loads, antigen retrieval protocols, and downstream detection systems.

    Handling, Reconstitution and Storage

    • Shipping: Distributed under temperature-controlled conditions on blue ice or dry ice to safeguard antibody binding integrity upon arrival.

    • Handling: Ready for immediate use. To minimize structural degradation or contamination risks, it is highly recommended to dispense the liquid solution into single-use working aliquots upon initial receipt.

    • Storage Framework:

      • Short-Term: Store at 4 degrees Celsius for up to 1 to 2 weeks.

      • Medium-Term: Store at -20 degrees Celsius for up to 12 months from the date of receipt.

      • Long-Term: Store at -80 degrees Celsius in a manual defrost freezer for maximum long-term structural stability.

      • Note: Avoid repeated freeze-thaw cycles to protect the monoclonal antibody conformation from performance loss.

Heading 6

Related Products

bottom of page