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Antibody System Anti-Neisseria meningitidis pilin protein Monoclonal Antibody (m

Antibody System Anti-Neisseria meningitidis pilin protein Monoclonal Antibody (m

Overview
This monoclonal antibody specifically targets the pilin protein of Neisseria meningitidis, universally recognized as the Putative Type IV Pilin Protein or NMO_0429. Type IV pili are filamentous surface appendages that play a foundational role in meningococcal pathogenesis, mediating initial host cell attachment, twitching motility, DNA transformation, and biofilm formation. Because these pilin structures are primary determinants of bacterial colonization across the nasopharyngeal epithelium and represent major targets for host immune recognition, they are focal points in vaccine development and virulence mapping. Generated from mouse hybridoma clone m1A03 using a recombinant pilin protein immunogen, this purified IgG2b monoclonal antibody delivers high-affinity target recognition specifically for Neisseria meningitidis (strain alpha14), providing a rigorous and highly reproducible tool for studying bacterial adhesion mechanics, surface antigen variations, and infectious disease kinetics.
 
DATASHEET

  • PRODUCT INFO

    Key Features and Specifications

    • Monoclonal Precision: Engineered from mouse clone m1A03 to ensure highly uniform binding kinetics, excellent signal-to-noise ratios, and absolute specificity for the meningococcal type IV pilin structural subunits.

    • Strain-Specific Validation: Characterized to target the surface pilin matrix of Neisseria meningitidis strain alpha14.

    • High Purity Profile: Refined via advanced Protein A/G affinity chromatography from cell culture supernatant to eliminate non-specific immunoglobulins and cultural contaminants.

    • Versatile Assay Performance: Validated and optimized across cornerstone bacteriology methodologies, including Western Blotting (WB) and Enzyme-Linked Immunosorbent Assays (ELISA).

    • High-Stability Liquid Matrix: Supplied as a ready-to-use liquid solution in a premium 0.01M PBS (pH 7.4) buffer fortified with 0.05% Proclin 300 to guarantee reliable, long-term shelf life.

    Technical Specifications

    • Catalog No.: MXX25501

    • Host Species: Mouse

    • Clone ID: m1A03

    • Clonality: Monoclonal

    • Isotype: IgG2b

    • Immunogen: Recombinant Neisseria meningitidis pilin protein

    • Accession Number: C6S5G8

    • Species Reactivity: Neisseria meningitidis (strain alpha14)

    • Purification: Protein A/G chromatography

    • Form: Liquid solution

    • Storage Buffer: 0.01M PBS, pH 7.4, 0.05% Proclin 300

    Alternative Names: Putative type IV pilin protein, pilin protein, NMO_0429, Neisseria type IV pili subunit.

    Applications and Recommended Dilutions

    This mouse monoclonal IgG2b antibody is highly suited for running quantitative, localized, and profile screens within bacterial pathogenesis and surface antigen mapping workflows. Investigators should utilize the following initial dilution guidelines:

    • Western Blotting (WB): 1:500 to 1:2000 dilution for evaluating target pilin subunits, structural assembly tracking, or expression levels in bacterial lysates and cell fractions.

    • Enzyme-Linked Immunosorbent Assay (ELISA): 1:1000 to 1:10000 dilution for target capture arrays, solid-phase sandwich screening assemblies, or antibody titration profiles.

    • Note: Optimal working dilutions must be determined empirically by the end-user according to specific sample antigen density, extraction protocols, and detection systems.

    Handling, Reconstitution and Storage

    • Shipping: Distributed under temperature-controlled conditions on blue ice or dry ice to safeguard antibody binding integrity upon arrival.

    • Handling: Ready for immediate use. To minimize structural degradation or contamination risks, it is highly recommended to dispense the liquid solution into single-use working aliquots upon initial receipt.

    • Storage Framework:

      • Short-Term: Store at 4 degrees Celsius for regular, frequent laboratory applications.

      • Long-Term: Store at -20 degrees Celsius in a manual defrost freezer for up to 12 months from the date of receipt.

      • ,Note: Avoid repeated freeze-thaw cycles to protect the monoclonal antibody conformation from performance loss.

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