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Antibody System Anti-HTLV-1 Glycoprotein 46 / gp46 Monoclonal Antibody (m1A05)

Antibody System Anti-HTLV-1 Glycoprotein 46 / gp46 Monoclonal Antibody (m1A05)

Overview
This monoclonal antibody specifically targets glycoprotein 46 (gp46), the major surface subunit of the envelope glycoprotein complex of Human T-cell Leukemia Virus 1 (HTLV-1). In the viral entry pathway, gp46 plays a foundational role by binding to the host cell surface receptors, such as GLUT1 and Neuropilin-1, initiating a conformational shift that allows the transmembrane subunit gp21 to mediate viral-host membrane fusion. Because it dictates viral infectivity and serves as a primary target for neutralizing antibody responses, gp46 is a key focal point in retroviral pathogenesis and diagnostics. Generated from mouse hybridoma clone m1A05 using a recombinant gp46 immunogen, this purified IgG monoclonal antibody delivers high-affinity, specific target recognition for HTLV-1 (strain Japan ATK-1 subtype A), offering a rigorous and highly reproducible tool for studying viral fusion mechanics, entry inhibition, and cellular tropism.
 
DATASHEET

  • PRODUCT INFO

    Key Features and Specifications

    • Monoclonal Precision: Engineered from mouse clone m1A05 to ensure highly uniform binding kinetics, excellent signal-to-noise ratios, and absolute specificity for the HTLV-1 gp46 envelope subunit.

    • Strain-Specific Validation: Characterized to target the envelope surface matrix of HTLV-1 strain Japan ATK-1 subtype A.

    • High Purity Profile: Refined via advanced Protein A/G affinity chromatography from cell culture supernatant to eliminate non-specific immunoglobulins and cultural contaminants.

    • Versatile Assay Performance: Validated and optimized across cornerstone virology methodologies, including Western Blotting (WB), Immunofluorescence (IF), and Enzyme-Linked Immunosorbent Assays (ELISA).

    • High-Stability Liquid Matrix: Supplied as a ready-to-use liquid solution in a premium 0.01M PBS (pH 7.4) buffer fortified with 0.05% Proclin 300 to guarantee reliable, long-term shelf life.

    Technical Specifications

    • Catalog No.: MVV4the 5301

    • Host Species: Mouse

    • Clone ID: m1A05

    • Clonality: Monoclonal

    • Isotype: IgG

    • Immunogen: Recombinant HTLV-1 Glycoprotein 46/gp46

    • Accession Number: P03381

    • Species Reactivity: Human T-cell leukemia virus 1 (HTLV-1)

    • Purification: Protein A/G chromatography

    • Form: Liquid solution

    • Storage Buffer: 0.01M PBS, pH 7.4, 0.05% Proclin 300

    Alternative Names: Glycoprotein 46, gp46, HTLV-1 Env surface protein, Retroviral envelope protein subunit gp46.

    Applications and Recommended Dilutions

    This mouse monoclonal IgG antibody is highly suited for running quantitative, localized, and profile screens within retroviral entry dynamics and expression mapping. Investigators should utilize the following initial guidelines:

    • Western Blotting (WB): Optimized for analyzing expression bands, structural protein processing, or viral particle presence in cell lysates, viral lysates, or supernatant fractions.

    • Immunofluorescence (IF): Tailored for high-resolution spatial mapping of viral envelope proteins on the surface of infected T cells or transfected expression models.

    • Enzyme-Linked Immunosorbent Assay (ELISA): Highly effective as a precise capture or detection layer for antigen quantification panels or envelope-binding kinetic screens.

    • Note: Optimal working dilutions must be determined empirically by the end-user according to specific sample viral loads, transfection efficiencies, and structural detection systems.

    Handling, Reconstitution and Storage

    • Shipping: Distributed under temperature-controlled conditions on blue ice or dry ice to safeguard antibody binding integrity upon arrival.

    • Handling: Ready for immediate use. To minimize structural degradation or contamination risks, it is highly recommended to dispense the liquid solution into single-use working aliquots upon initial receipt.

    • Storage Framework:

      • Short-Term: Store at 4 degrees Celsius for regular, frequent laboratory applications.

      • Long-Term: Store at -20 degrees Celsius in a manual defrost freezer for up to 12 months from the date of receipt.,

      • Note: Avoid repeated freeze-thaw cycles to protect the monoclonal antibody conformation from performance loss.

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