Logos Yeast Viability Kit 1
Yeast Viability Kit 1 is used to analyze the cell concentration and viability of yeast samples with the automated fluorescence cell counters of the LUNA™ family.
Fluorescein Diacetate Stain and Propidium Iodide Stain for Yeast are used to assess cell viability. Viable cells hydrolyze fluorescein diacetate into the green fluorescent compound fluorescein, causing viable cells to fluoresce green. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes binding to the nucleus of nonviable cells and causing them to fluoresce red.
Fluorescence Signal Enhancer 1 is an inhibitor of ATP-dependent fluorescein efflux. By blocking fluorescein from being pumped out of the cell, Fluorescence Signal Enhancer 1 lowers background and increases signal intensity, thereby enhancing the fluorescein signalto-noise ratio. Fluorescence Signal Enhancer 1 is most effective with metabolically active cells in the log growth phase. Cell Dilution Buffer is for washing cells and diluting cell suspensions.
Product Info
Compatible Instruments
LUNA-FL™ Automated Cell Counter
LUNA-II YF™ Automated Yeast Cell Counter
LUNA-FX7™ Automated Cell CounterSpecifications
Fluorescein Diacetate Stain Molecular formula C24H16O7 Molecular weight 416.38 g/mol Appearance Clear liquid Cell permeability Membrane permeant Excitation/emission 494/521 nm (when converted to fluorescein) Quantity 1 x 0.5 mL Storage -20°C in the dark Propidium Iodide for Yeast Molecular formula C27H34I2N4 Molecular weight 668.39 g/mol Appearance Clear liquid Cell permeability Membrane impermeant Excitation/emission 493/636 nm (in aqueous solution)
533/617 nm (when bound to nucleic acids)Quantity 1 x 0.5 mL Storage 4°C in the dark Fluorescence Signal Enhancer 1 Appearance Clear liquid Quantity 1 x 0.5 mL Storage -20°C in the dark Cell Dilution Buffer Appearance Clear liquid Quantity 1 x 20 mL Storage Room temperature Ordering Information
Cat # Description Qty F23202 Yeast Viability Kit 1 1 Kit
